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RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 81-86 doi: 10.15302/J-FASE-2016088

摘要： As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

关键词： pseudorabies virus     RR1 and RR2     ribonucleotide reductase     vaccine candidate    

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Purification and characterization of a novel carbonyl reductase with high stereo-selectivity

YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong

《化学科学与工程前沿（英文）》 2007年 第1卷 第4期   页码 404-410 doi: 10.1007/s11705-007-0074-9

摘要： A novel NADPH-dependent carbonyl reductase was separated from CCTCC 203011. The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which was purified through ammonium sulfate, Diethylamino Ethanol (DEAE) sepharose Fast flow (FF), phenyl-sepharose FF and blue sepharose FF chromato graphy from cell-free extract. The molecular mass of the enzyme was about 30 kDa. The optimum pH and temperature for reduction were 4.5°C and 35°C, respectively. The Cu had strong restrictive effect on enzyme activity. In addition, the carbonyl reductase was an enzyme with high substrate specificity and stereo-selectivity, and showed high asymmetric reduction activity towards -hydroxyacetophenone and ethyl 4-chloro acetoacetate. For the asymmetric reduction of ?-hydroxyacetophenone and ethyl 4-chloro acetoacetate, ()-1-phenyl-1,2-ethanediol and ()-ethyl 4-chloro-3-hydroxybutanoate were produced by the purified enzyme, with the 100% and 94.3% e.e. value, respectively. Therefore, the enzyme could be one of the effective biocatalysts for asymmetric synthesis of chiral alcohols. The amino acid sequences of one peptide from the purified enzyme were analyzed by LC-MASS-MASS, and the carbonyl reductase showed some identity to the hypothetical protein CaO19.10414 reported.

关键词： asymmetric synthesis     sulfate-polyacrylamide     purified     electrophoresis     temperature    

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A novel aldo-keto reductase gene,

Jinxi HUO, Bing DU, Sifan SUN, Shaozhen HE, Ning ZHAO, Qingchang LIU, Hong ZHAI

《农业科学与工程前沿（英文）》 2018年 第5卷 第2期   页码 206-213 doi: 10.15302/J-FASE-2018225

摘要： High concentrations of Cd can inhibit growth and reduce the activity of the photosynthetic apparatus in plants. In several plant species, aldo-keto reductases (AKRs) have been shown to enhance tolerance to various abiotic stresses by scavenging cytotoxic aldehydes; however, few AKRs have been reported to enhance Cd stress tolerance. In this study, the gene was isolated from sweet potato. The relative expression levels of increased significantly (approximately 3-fold) after exposure to 200 mol·L CdCl or 10 mmol·L H O . A subcellular localization assay showed that is predominantly located in the nucleus and cytoplasm. -overexpressing tobacco plants showed higher tolerance to Cd stress than wild-type (WT). Transgenic lines showed a significant ability to scavenge malondialdehyde (MDA) and methylglyoxal (MG). In addition, proline content and superoxide dismutase activity were significantly higher and H O levels were significantly lower in the transgenic plants than in the WT. Quantitative real-time PCR analysis showed that the reactive oxygen species (ROS) scavenging genes encoding guaiacol peroxidase ( ), ascorbate peroxidase ( ), monodehydroascorbate reductase ( ) and peroxidase ( ) were significantly upregulated in transgenic plants compared to WT under Cd stress. These findings suggest that overexpressing enhances tolerance to Cd stress via the scavenging of cytotoxic aldehydes and the activation of the ROS scavenging system.

关键词： cadmium stress     IbAKR     Ipomoea batatas     sweet potato    

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Long-term effects of various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes

Seo Yeon Baik, Hyunah Kim, So Jung Yang, Tong Min Kim, Seung-Hwan Lee, Jae Hyoung Cho, Hyunyong Lee, Hyeon Woo Yim, Kun-Ho Yoon, Hun-Sung Kim

《医学前沿（英文）》 2019年 第13卷 第6期   页码 713-722 doi: 10.1007/s11684-018-0661-9

摘要： Few long-term follow-up studies have compared the changes in renal function according to the type of statin used in Korea. We compared the long-term effects of statin intensity and type on the changes in the glomerular filtration rate (GFR). We extracted data of patients who took statin for the first time. We analyzed whether or not different statins affect the changes in GFR at 3 months after baseline and 4 years after. We included 3678 patients and analyzed the changes in GFR. The GFR decreased by 3.2%±0.4% on average 4 years after the first statin prescription, indicating statistically significant deterioration (from 83.5±0.4 mL/min/1.73 m to 79.9±0.4 mL/min/1.73 m , <0.001). When comparing the GFR among different statins, significant differences were observed between atorvastatin and fluvastatin (−5.3%±0.7% vs. 1.2%±2.2%, <0.05) and between atorvastatin and simvastatin (−5.3%±0.7% vs. −0.7%±0.8%, <0.05). In pitavastatin (odds ratio [OR]=0.64, 95% confidence interval [CI]=0.46–0.87, <0.005) and simvastatin (OR=0.69, 95% CI=0.53–0.91, <0.008), the GFR rate that decreased by<60 mL/min/1.73 m was significantly lower than that of atorvastatin. Regarding long-term statin intake, GFR changed with the type of statin. This work is the first in Korea to compare each statin in terms of changes in the GFR after the statin prescription.

关键词： statin     glomerular filtration rate     HMG-CoA reductase inhibitor     chronic kidney disease    

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Phylogenetic diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered environments

《环境科学与工程前沿（英文）》 2022年 第16卷 第10期 doi: 10.1007/s11783-022-1562-3

摘要：

● 548 representative nor genes were collected to create complete phylogenetic trees.

关键词： N2O     Greenhouse gas     NO reductase     NO dismutase     Primer     Crystal structure    

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Decolorization of azo dyes by a salt-tolerant

Bin YAN, Cuihong DU, Meilan XU, Wenchao LIAO

《环境科学与工程前沿（英文）》 2012年 第6卷 第6期   页码 806-814 doi: 10.1007/s11783-012-0453-4

摘要： The salt-tolerant strain, isolated from textile wastewater, has been found effective on decolorizing several kinds of azo dyes with different structures. The optimal conditions for azo dye acid red B (ARB) decolorization by were determined to be pH= 7.0 and 30°C. The decolorization efficiency increased with the increase of the salinity concentration, and around 90% of ARB (100 mg·L ) could be decolorized in 24 h when the salinity concentration was up to 50 g·L . Moreover, the strain could still decolorize 19% of ARB in 24 h even when the NaCl concentration was increased to 150 g·L . Meanwhile, the dependence of the specific decolorization rate by on the ARB concentration could be described with Michaelis-Menten kinetics ( = 585.7 mg·L , = 109.8 mg·g cell ·h ). The addition of quinone redox mediator, named 2-hydroxy-1,4-naphthoquinone and anthraquinone-2,6-disulfonate, significantly accelerated the decolorization performance of . Furtherly, the activities of azoreductase (0.55 μmol·mg protein ·min ) and Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase (8.9 μmol·mg protein ·min ) have been observed in the crude cell extracts of . The decolorization products of ARB were analyzed by HPLC-MS, and the results indicated the reductive pathway was responsible for azo dye decolorization by .

关键词： Staphylococcus cohnii     decolorization     salt     azoreductase     Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase    

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标题 作者 时间 类型 操作

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

期刊论文

Purification and characterization of a novel carbonyl reductase with high stereo-selectivity

YANG Ming, XU Yan, MU Xiaoqing, XIAO Rong

期刊论文

A novel aldo-keto reductase gene,

Jinxi HUO, Bing DU, Sifan SUN, Shaozhen HE, Ning ZHAO, Qingchang LIU, Hong ZHAI

期刊论文

Long-term effects of various types of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on changes

Seo Yeon Baik, Hyunah Kim, So Jung Yang, Tong Min Kim, Seung-Hwan Lee, Jae Hyoung Cho, Hyunyong Lee, Hyeon Woo Yim, Kun-Ho Yoon, Hun-Sung Kim

期刊论文

Phylogenetic diversity of NO reductases, new tools for monitoring, and insights into NO production in natural and engineered environments

期刊论文

Decolorization of azo dyes by a salt-tolerant

Bin YAN, Cuihong DU, Meilan XU, Wenchao LIAO

期刊论文